VM 527-Centrifugation and Aliquoting of Blood Serum and Plasma

This video shows Dr. Evan Matthews explaining how to centrifuge blood samples and use transfer pipettes to remove the serum or plasma for immediate use or storage.

Link to video showing how to use transfer and precision pipettes.
https://youtu.be/3d-U9vowVwg

Link to video showing how to use a microcentrifuge.
https://youtu.be/PNhDbPCPHCs


Link to Dr. Evan Matthews website.
https://sites.google.com/site/evanmatthewseportfolio/home


I attempt to make the most accurate material I can. However, mistakes happen. Below is a list of mistakes identified in this video.

Known errors in this video:

At 1:34 I state that plasma still contains platelets after centrifugation. This is generally not true. The platelets settle into the thin white "buffy coat" that also contains the white blood cells. You can alter the process to create a platelet rich plasma. I have links below on how that works.
See my error:
https://youtu.be/XAhBzUosvsU?t=93
How to make platelet rich plasma:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338460/
https://www.hindawi.com/journals/isrn/2014/176060/
Discussion of the buffy coat:
https://onlinelibrary.wiley.com/doi/abs/10.1046/j.1537-2995.1990.30790385523.x